USGS - science for a changing world

National Water Quality Laboratory

Friday December 15, 2017

USGS Open-File Report 96-216

Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory — Determination of Pesticides in Water by Carbopak-B Solid-Phase Extraction and High-Performance Liquid Chromatography

Stephen L. Werner, Mark R. Burkhardt, and Sabrina N. DeRusseau


In accordance with the needs of the National Water-Quality Assessment Program (NAWQA), the U.S. Geological Survey has developed and implemented a graphitized carbon-based solid-phase extraction and high-performance liquid chromatographic analytical method. The method is used to determine 41 pesticides and pesticide metabolites that are not readily amenable to gas chromatography or other high-temperature analytical techniques. Pesticides are extracted from filtered environmental water samples using a 0.5-gram graphitized carbon-based solid-phase cartridge, eluted from the cartridge into two analytical fractions, and analyzed using high-performance liquid chromatography with photodiode-array detection. The upper concentration limit is 1.6 micrograms per liter (µg/L) for most compounds. Single-operator method detection limits in organic-free water samples ranged from 0.006 to 0.032 µg/L. Recoveries in organic-free water samples ranged from 37 to 88 percent. Recoveries in ground- and surface-water samples ranged from 29 to 94 percent. An optional on-site extraction procedure allows for samples to be collected and processed at remote sites where it is difficult to ship samples to the laboratory within the recommended pre-extraction holding time of 7 days.

VIEW the report. (pdf)

Adobe ® Portable Document Files (pdfs), Microsoft Powerpoint ® Files and Other Sites Open in a New Window

NELAC logo TNI logo NWQL logo

Accessibility FOIA Privacy Policies and Notices

Take Pride in America logo logo U.S. Department of the Interior | U.S. Geological Survey
Page Contact Information: NWQL LabHelp
Page Last Modified: Sunday, 16-Apr-2017 19:55:12 MDT